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Image Search Results
Journal: iScience
Article Title: Group 3 innate lymphocytes make a distinct contribution to type 17 immunity in bladder defence
doi: 10.1016/j.isci.2022.104660
Figure Lengend Snippet:
Article Snippet: Mouse antibody : anti-F4/80- AF647 (ab204467, Abcam);
Techniques: Recombinant, Protease Inhibitor, Blocking Assay, Expressing, Enzyme-linked Immunosorbent Assay, Multiplex Assay, RNA Sequencing Assay, Infection, Knock-Out, Software, Spectrophotometry, Microscopy
Journal: Frontiers in Immunology
Article Title: Mfn2 is responsible for inhibition of the RIG-I/IRF7 pathway and activation of NLRP3 inflammasome in Seneca Valley virus-infected PK-15 cells to promote viral replication
doi: 10.3389/fimmu.2022.955671
Figure Lengend Snippet: SVV decreased Mfn2 expression, inhibited NLRP3 inflammasome activation. (A–C) The western blot assay for Mfn2, NLRP3, ASC, caspase1/p10/p20, cleaved-IL-1β and IL-18. (D–I) The relative protein expression level of Mfn2, NLRP3, ASC, caspase1/p10/p20, cleaved-IL-1β and IL-18. Unpaired T-test were used to investigate the significance of differences between the experimental groups and the control group *; P < 0.05, ***; P < 0.005,.
Article Snippet: The primary antibodies were RIG-I polyclonal antibody (CST, United States), anti-Phospho-IRF7 (Bioss, China), GAPDH (Abcam, United States),
Techniques: Expressing, Activation Assay, Western Blot
Journal: Frontiers in Immunology
Article Title: Mfn2 is responsible for inhibition of the RIG-I/IRF7 pathway and activation of NLRP3 inflammasome in Seneca Valley virus-infected PK-15 cells to promote viral replication
doi: 10.3389/fimmu.2022.955671
Figure Lengend Snippet: NLRP3 inflammasome activator (Nigeration) relieved SVV inhibited NLRP3 inflammasome activation and suppress SVV proliferation in PK-15 cells (A) The western blot assay for NLRP3, ASC, caspase1/p10/p20, cleaved-IL-1β and IL-18. (B–F) The relative protein expression level of NLRP3, ASC, caspase1/p10/p20, cleaved-IL-1β and IL-18. (G) The qRT-PCR assay for SVV copy number. One-way analysis of variance (ANOVA) were used to investigate the significance of differences between the SVV groups and the SVV+BX795 group or between the control group and SVV group *; P < 0.05, **; P < 0.01, ***; P < 0.005.
Article Snippet: The primary antibodies were RIG-I polyclonal antibody (CST, United States), anti-Phospho-IRF7 (Bioss, China), GAPDH (Abcam, United States),
Techniques: Activation Assay, Western Blot, Expressing, Quantitative RT-PCR
Journal: Frontiers in Immunology
Article Title: Mfn2 is responsible for inhibition of the RIG-I/IRF7 pathway and activation of NLRP3 inflammasome in Seneca Valley virus-infected PK-15 cells to promote viral replication
doi: 10.3389/fimmu.2022.955671
Figure Lengend Snippet: Mfn2 overexpression induced SVV-inhibited NLRP3 inflammasome activation in PK-15 cells. (A) The western blot assay for NLRP3, ASC, caspase1/p10/p20, cleaved-IL-1β and IL-18. (B–F) The relative protein expression level of NLRP3, ASC, caspase1/p10/p20, cleaved-IL-1β and IL-18. One-way analysis of variance (ANOVA) were used to investigate the significance of differences. * means the difference between the WT and Ad-Mfn2 in control group; # means the difference between the WT and Ad-Mfn2 in SVV group; and other groups. ## ; P < 0.01, ### ; P < 0.005, **** ,#### ; P < 0.001, ns means no significance.
Article Snippet: The primary antibodies were RIG-I polyclonal antibody (CST, United States), anti-Phospho-IRF7 (Bioss, China), GAPDH (Abcam, United States),
Techniques: Over Expression, Activation Assay, Western Blot, Expressing